https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Cooperativity of HOXA5 and STAT3 is critical for HDAC8 inhibition-mediated transcriptional Activation of PD-L1 in human melanoma cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32982 Wed 17 Nov 2021 16:28:27 AEDT ]]> MEK-independent survival of B-RAFV600E melanoma cells selected for resistance to apoptosis induced by the RAF inhibitor PLX4720 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15166 V600E melanoma cells to B-RAF inhibitors. Experimental Design: B-RAFV600E melanoma cells were exposed to the B-RAF inhibitor PLX4720 for prolonged periods to select for cells resistant to apoptosis induced by the inhibitor. The resultant cells were analyzed for activation of extracellular signal regulated kinase (ERK), MAP/ERK kinase (MEK), and Akt, and related signals. Their roles in survival of the cells were also examined. Results: B-RAFV600E melanoma cells selected for resistant to PLX4720-induced apoptosis retained the V600E mutation in B-RAF, and proliferated steadily in the presence of the inhibitor, albeit with slow growth rate. These cells displayed high levels of ERK activation, that is, at least in part, independent of the conventional RAF/MEK/ERK pathway, as MEK activation was low and inhibition of MEK did not significantly block activation of ERK. In contrast, extracellular signals appeared involved. This was associated with elevated activation of the phosphoinositide 3-kinase (PI3k)/Akt pathway and could be inhibited by serum starvation and inhibition of PI3k/Akt. Inhibition of MEK did not impact on survival of these cells, whereas serum starvation, inhibition of PI3K/Akt, and inhibition of ERK1/2 reduced their viability. Conclusions: These results indicate that sensitivity to induction of apoptosis may be a major determinant of long-term responses of B-RAFV600E melanomas to specific inhibitors and suggest that rebound melanoma growth after initial treatment with the inhibitors may not be responsive to MEK inhibitors, but may be susceptible to inhibition of the PI3k/Akt pathway.]]> Wed 11 Apr 2018 16:15:39 AEST ]]> Oncogenic activation of MEK/ERK primes melanoma cells for adaptation to endoplasmic reticulum stress https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14866 Wed 11 Apr 2018 16:08:27 AEST ]]> 2-Deoxy-D-glucose enhances TRAIL-induced apoptosis in human melanoma cells through XBP-1-mediated up-regulation of TRAIL-R2 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:6913 Wed 11 Apr 2018 15:38:21 AEST ]]> Reactive oxygen species dictate the apoptotic response of melanoma cells to TH588 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28216 Wed 11 Apr 2018 09:38:40 AEST ]]> Skp2-mediated stabilization of MTH1 promotes survival of melanoma cells upon oxidative stress https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32181 Wed 09 Mar 2022 15:58:36 AEDT ]]> Human melanoma cells selected for resistance to apoptosis by prolonged exposure to tumor necrosis factor-related apoptosis-inducing ligand are more vulnerable to necrotic cell death induced by cisplatin https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:999 Sat 24 Mar 2018 08:29:51 AEDT ]]> Contrasting effects of Nutlin-3 on TRAIL- and Docetaxel-induced Apoptosis due to upregulation of TRAIL-R2 and Mcl-1 in human melanoma cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:10447 Sat 24 Mar 2018 08:08:03 AEDT ]]> Quantification of hTERT splice variants in melanoma by SYBR green real-time polymerase chain reaction indicates a negative regulatory role for the β deletion variant https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:5432 Sat 24 Mar 2018 07:48:16 AEDT ]]> Targeting human MutT homolog 1 (MTH1) in melanoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32005 Fri 10 Aug 2018 15:21:11 AEST ]]>